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bca protein assay kit  (Bio-Rad)


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    Structured Review

    Bio-Rad bca protein assay kit
    Bca Protein Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 41119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+protein+assay+kit/Bio-Rad+Protein+Assay+Kit+I/pmc13098594-60-7-11
    Average 97 stars, based on 41119 article reviews
    bca protein assay kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Protein Concentration:

    Article Title: Discovery of a CI-994 derivative as a dual modulator of class I HDACs and Wnt/β-catenin signaling for Alzheimer’s disease therapy
    Article Snippet: .. Supernatants were collected, and total protein concentration was measured using a Bio-Rad Protein Assay Kit (Bio-Rad, cat. no. 5000002EDU). ..

    Article Title: The Antidepressant Amitriptyline Upregulates ERK1/2 Signaling and Inhibits Rho-Mediated Responses Induced by Lysophosphatidic Acid in Astroglial Cells.
    Article Snippet: Following treatment, cells were washed in ice-cold phosphate-buffered saline (PBS) (pH 7.4) and scraped in ice-cold RIPA buffer containing PBS, 4 mM sodium pyrophosphate, 2 mM sodium orthovanadate, 10 mM sodium fluoride, 20 nM okadaic acid, 0.5% phosphatase inhibitor cocktail 3, 1% protease inhibitor cocktail (Sigma Aldrich), 1 mM PMSF, 0.1% sodium dodecyl sulphate (SDS), 1% Nonidet P-40 and 0.5% sodium deoxycholate (pH 7.4). .. Cell lysates were subjected to sonication for 5 s at ice-bath temperature, and the protein concentration was determined by the Bio-Rad Protein assay kit (Bio-Rad Lab., Hercules, CA, USA). .. Aliquots of the cell lysates containing an equal amount of protein (10–15 μg/lane) were mixed with 5x Laemmli sample buffer and loaded onto either homemade 10 and 12.5% polyacrylamide gels or pre-casted Nu-PAGE Bis-Tris gels (10 and 4–12%, Invitrogen/Thermo Fisher Scientific, Monza, Italy).

    Article Title: A Zebrafish Galectin-1 Isoform Is Expressed in Skin and Gills and Binds to Bacteria, Bacterial Adhesin Receptors, and Epidermal Mucus Glycans.
    Article Snippet: .. Samples were quantified for protein concentration using a Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: Maternal Infection Impairs Motor Coordination in an Experimental Meningitis Rat Model Through Altered MMP-2/3/9 Activity, H3K4 Trimethylation, and Reln Methylation.
    Article Snippet: .. Protein concentration was quantified (Cat. #5000006; Bio-Rad Protein Assay Kit) and stored at −80 ◦C [28]. ..

    Sonication:

    Article Title: The Antidepressant Amitriptyline Upregulates ERK1/2 Signaling and Inhibits Rho-Mediated Responses Induced by Lysophosphatidic Acid in Astroglial Cells.
    Article Snippet: Following treatment, cells were washed in ice-cold phosphate-buffered saline (PBS) (pH 7.4) and scraped in ice-cold RIPA buffer containing PBS, 4 mM sodium pyrophosphate, 2 mM sodium orthovanadate, 10 mM sodium fluoride, 20 nM okadaic acid, 0.5% phosphatase inhibitor cocktail 3, 1% protease inhibitor cocktail (Sigma Aldrich), 1 mM PMSF, 0.1% sodium dodecyl sulphate (SDS), 1% Nonidet P-40 and 0.5% sodium deoxycholate (pH 7.4). .. Cell lysates were subjected to sonication for 5 s at ice-bath temperature, and the protein concentration was determined by the Bio-Rad Protein assay kit (Bio-Rad Lab., Hercules, CA, USA). .. Aliquots of the cell lysates containing an equal amount of protein (10–15 μg/lane) were mixed with 5x Laemmli sample buffer and loaded onto either homemade 10 and 12.5% polyacrylamide gels or pre-casted Nu-PAGE Bis-Tris gels (10 and 4–12%, Invitrogen/Thermo Fisher Scientific, Monza, Italy).

    Spectrophotometry:

    Article Title: Bioaccessibility of B vitamins in faba bean extrudates: Influence of protein ingredients and added ascorbic acid in high-moisture extrusion
    Article Snippet: .. The soluble proteins were determined using a Bio-Rad Protein Assay kit with a microplate spectrophotometer (650 nm). ..



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